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Sperm samples were centrifuged through a PureSperm 80/40 (Nidacon, Gothenburg, Sweden) gradient at at 1,500 rpm for 8 min

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4.2 Immune checkpoint inhibitors Checkpoint receptors on the surface of T cells, such as PD-1 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), can suppress energy and metabolic changes in T cells and mediate immunosuppression when activated by the corresponding ligands (160)

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