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Cell pellets were resupended in ice-cold buffer 1 containing 20 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C and stirred on ice with DNase I and 10 mg/mL lysozyme for 1 h before sonication

For most people yes

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In an isolated context, this might seem beneficial for someone experiencing retatrutide-induced appetite suppression