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Quantitative real-time PCR (qRT-PCR) was performed using SYBR Green PCR master mix (Roche Applied Science, Mannheim, Germany)

In addition to the gut-brain and gut-kidney axes, the gut microbiota systemically communicates through other axes such as the gut-liver (Albillos et al., 2020), gut-heart (Zhao et al., 2023), gut-skin (Sinha et al., 2021), and gut-bone (Zhang et al., 2024) axes, among others

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It begins attacking the stomach lining as if it were a threat