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To obtain pgfp, a gfp containing fragment was released from pUCG-H [34] via Sma I and Sac I and subcloned into the corresponding Eco RV and Sac I sites of pGEM5zf+ (Promega)

A: No, it does not show direct cause-and-effect

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Ipamorelin is a selective growth hormone secretagogue that triggers GH release through a different (ghrelin-receptor) pathway

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