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After quantification using the BCA protein assay kit, sampling buffer was added to the supernatant, which was mixed and boiled for 10 min

Manea S-A, Antonescu M-L, Fenyo IM, Raicu M, Simionescu M, Manea A

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However, the kinetic patterns do not support a ping-pong mechanism and the nonenzymatic first reaction with a second order rate constant of 0.6 M 1 s 1 is too slow to explain the rapid turnover 3,12,14,15,30

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