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Glucagon-like peptide-1 receptor agonists (GLP-1 agonists), such as semaglutide (Ozempic, Rybelsus, Wegovy), have helped improve the management of type 2 diabetes and obesity

Fixed cells were incubated in blocking buffer containing PBTA (0.5% BSA and 0.1% Triton X100 in PBS) plus 5% normal donkey serum for 1 h at room temperature

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These effects are usually mild to moderate and often become less noticeable over time

The brain is remarkably sensitive to peptide signaling, which is why fatigue can be such a prominent early effect of GLP-1 agonist therapy

Hence, it is critical to gain insights specifically into the mechanisms underpinning any effects of GLP-1R agonists in preclinical models of lipodystrophy to better understand their use for treating patients with these conditions