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Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 g/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 g/ml SeNPs (GSH + SeNPs group)

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These targets can not only be used to identify tumors that may be sensitive to inhibitors but also offer the potential for combining kynurenine pathway inhibition with immune checkpoint blockade as a promising approach to treating resistant cancers

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