Funding This work was supported by the National Natural Science Foundation of China (32102293), the National Key Laboratory of Green Pesticide, Guizhou University (GPLKF202506), the Carbon Peak Carbon Neutral Science and Technology Innovation Special Fund of Jiangsu Province (BE2022424), the Jiangsu Agricultural Science and Technology Innovation Fund (CX(24)2008 and CX(22)1001), the Earmarked Fund for CARS (Rice, CARS-01-26), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX23_3578 and SJCX24_2276), the College Students Innovative Entrepreneurial Training Plan Program (202411117194Y), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
[Abstract] Chin J Nat Med
For melasma and hyperpigmentation specifically, consistency matters most
For cell proliferation assays with fold change in cell number, cells were cultured in replicates of 3 in 96-well plates at 1000 (suspension) or 150 (adherent) cells per well in 200 uL RPMI or DMEM base media under the conditions described in each experiment, and a separate group of 3 wells was also plated for each cell line with no treatment for an initial time point
Many of the mechanisms have been elucidated by the Jaeschke group in Kansas [18,36,37]
UMAPs stratified by lesion type revealed shifts in microglia, astrocyte, and MOL transcriptional states (Fig