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If your refrigerator has cold spots near the freezer compartment, or if you accidentally placed a vial in the freezer and then thawed it, the damage from a single freeze-thaw event can reduce potency by 30-50% or more

2021 Feb 2;25(1):76-80

doi: 10.1056/NEJMoa2314076 73 MaCHoqueSSparrowMPAndersonJKYenMDongBet al

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Abstract Circadian clocks are internal timekeepers enabling organisms to adapt to recurrent events in their environment such as the succession of day and nightby controlling essential behaviors such as food intake or the sleep-wake cycle

Documentation of reaction patterns, including photographs if possible, helps clinicians assess severity and guide management decisions