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Structural alignment of the ligand-binding pockets for the two agonists and the antagonist C12 suggests that agonist-binding causes a subtle change in the salt bridge between E124 3.33 and R283 6.55 (Fig

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In contrast, indicator systems that specifically sense particular redox couples allow real-time observation of redox changes (Gutscher et al., The experimental difficulties to obtain reliable potentials of thiol/disulfide systems prompt further concern to accept these parameters or changes thereof as critical determinants of biological events

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