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Non-canonical activation mechanisms may exist and the ECD could play a different role assuming different conformations depending on the ligand 24

Given the limited efficacy of current anti-fibrotic therapies and the substantial morbidity associated with progressive pulmonary fibrosis, identification of novel pathways capable of modulating fibroblast activation and ECM remodelling remains a major therapeutic priority

Glycerol is then oxidized to generate a product that reacts with a probe to generate fluorescence when excited at 535 nm