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Sulforaphane was found to attenuate pancreatic injury in a mouse model of acute pancreatitis by stimulating Nrf2-induced antioxidant enzymes (18)

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In contrast to the Kataoka's mice model where PLC was expressed in a shortened and catalytically inactive form, the knockout models described by Martins and coauthors either have a complete loss of the PLC expression (PLC -/- ) or have expression of the mutant form of PLC with mutant RA domains enable to bind to Ras (PLC RAm/RAm )

Protein expression in BL21 was induced with 0.4 mM IPTG when the cultures reached an OD 600 of 0.60.8 at 37 C in LB medium and then incubated for 20 h at 20 C after induction