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M9 minimal salt medium containing 5 g/L glucose, 15.1 g/L Na 2 HPO 4 , 3 g/L KH 2 PO 4 , 0.5 g/L NaCl, 1 g/L NH 4 Cl, 0.5 g/L MgSO 4 7H 2 O, 0.01 g/L CaCl 2 , 0.2 mL/L 1 % vitamin B1 and a 0.05 mL/L stock solution of trace elements was used for the cultivation of engineered E
Neither format is universally superior
Draw the intended dose, then check for air bubbles and push any out
2016, pp
The daily requirement of the human body for Vitamin B12 is about 3 micrograms
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