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Oxidation occurs when certain amino acid side chains (methionine, cysteine, and tryptophan are particularly susceptible) react with oxygen or light-generated reactive species, altering the peptide's structure and often its biological activity

While freezing is an option for very long-term storage (many months to years), for most research timelines, refrigeration is perfectly sufficient.

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Normalization was performed iteratively (across samples and spectra) on intensities, as described in Statistical Analysis of Relative Labeled Mass Spectrometry Data from Complex Samples using ANOVA 22

Melanotan is a synthetic peptide a short chain of amino acids

As glutathione levels fall due to apoptosis, the oxidized form of gliotoxin effluxes from the cell where the cytocidal effects of gliotoxin are perpetuated in a pseudocatalytic manner