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The low GSH concentration necessary for CVA21 displacement may also limit a co-elution of potentially bound host cell GST enzymes present in cell culture harvests, which may require as high as 10 mM GSH for elution (Figure 4A) and elution NaCl gradient (Supplementary Figure S6C) studies where CVA21 particles eluted at lower ionic strengths with increasing pH

Bjrnsson ES, Bergmann OM, Bjrnsson HK, Kvaran RB, Olafsson S

L-Acetylcarnitine causes analgesia in mice modeling Fabry disease by up-regulating type-2 metabotropic glutamate receptors