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RNA isolation and quantitative RT-PCR gene expression levels The total RNA from the kidney tissue was isolated using the TRIzol reagent (Invitrogen) [27], and the cDNA was synthesized following the manufacturers protocol, using 1 g RNA (Prime ScriptTM RT reagent Kit, Takara)

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Lee, MD, MPH, a professor of pediatrics and communicable diseases at the University of Michigan School of Public Health, and colleagues, interest in GLP-1 receptor agonists have soared in the past decade, especially after the 2021 approval of semaglutide (Wegovy, Novo Nordisk) for weight management in adults

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Central to these effects is the modulation of glucose metabolism in brain regions associated with emotional and cognitive functions, suggesting a potential role for energy homeostasis in mental health

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