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Increased platelet activation in -thalassemia/HbE patients By using flow cytometry, the isolated platelet populations were analyzed using plots of forward scatter (FSC-H) and side scatter (SSC-H)

Shi Q, Xie Y, Wang Y, Li S

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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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