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282 The resulting amplification loop, involving neutrophil- and macrophage-derived TNF-, intensifies the local inflammatory response in the pancreas

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Cryo-CLEM INS-1 832/3 SNAP/FLAG-hGLP-1R cells previously transfected with EGFP-VAPB were plated onto glow discharged (30 seconds, 20 mAmp) poly-L-lysine-coated Quantifoil R2/2 200 mesh gold EM grids (Electron Microscopy Sciences)