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The proposed mechanism is indirect modulation of the NO pathway rather than direct receptor agonism, though the precise molecular binding event has not been definitively characterized in peer-reviewed work

Remember to add controls (not included in this kit)* Add 75 microliters of Elution Buffer in each well, mix well the contents of each well and place the plate in a plate shaker (preferably in an incubator at 37 degrees C) Wait 20 minutes for dried blood spots or 8 minutes for whole blood samples

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Gentle Mixing: Once all the water is added, don't shake the vial vigorously