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For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid

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Concurrent alcohol intake lowers these thresholds significantly

Despite this modification, about 85% of NAC still undergoes oxidation, which can lead to unwanted side effects like gastrointestinal distress