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The cells treated for 1 h with 200 M of the ROS inducer TBHP were used as a positive control

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Despite this short plasma half-life, the downstream GH pulse it triggers lasts 2-3 hours, and the resulting IGF-1 elevation persists for 12-18 hours

Subsequently, TBS-protected phenylhydrazine- N -hydroxysuccinimide ester (compound 7) DMSO solution (100 mM, 5 l) was slowly added to the above buffer and incubated for 2 h at 37 C on a thermostatic mixer

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