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Reviewer #1 The present manuscript describes the role of the GHSR in intracellular signaling on its own and in a variety of molecular interactions with other receptors as well as the potential for stimulation of GHSRs as a therapeutic target for a wide-range of clinical conditions

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Rodrigues AR, Almeida H, Gouveia AM

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Transwell membranes were pre-coated with 1% Matrigel in cold ISC basal medium and incubated for 1 hour at 37 C