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Given that bitter herbs have evidently been used in times of scarcity to reduce hunger, researchers tested low-and-high-doses of hops extracts on people undergoing a 24-hour fast, and found both doses seemed to lower feelings of hunger better than placebo
Samples (20 l) were mixed with 5 l of 5x loading buffer (0.3 M Tris-HCl pH = 6.8, 25% -mercaptoethanol, 50% glycerol, 10% SDS, 1% bromophenol blue), heated to 95 C for 5 min, and 15 l of samples were applied to the gel together with 5 l of Thermo Scientific PageRuler Unstained Broad Range Protein Ladder
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10.1111/nep.13114 15 RoffeyS