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The results from the Boston collaborators raised questions about the physiological way that GLP-1 prompted the release of insulin, a phenomenon described as an 'insulinotropic effect' (Drucker, Philippe, Mojsov)

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For these assays, increased levels of cAMP were measured as an increase of the 485/535 nm FRET ratio serving as a readout for binding of cAMP to the H188 biosensor that is based on the exchange protein activated by cAMP 15

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The freeze-drying process removes water, and without water, the chemical reactions that break down peptide bonds essentially stop