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qPCR conditions were: initial heat activation at 95 C for 10 min, followed by 40 cycles of alternating between 95 C for 15 s, and 60 C for 60 s

PMID 22221769

CHAC1s expression is rapidly upregulated upon ferroptosis induction by agents such as erastin

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It is primarily found in animal-based foods, such as meat, fish, eggs, and dairy products