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After filtering out low quality cells based on total UMI counts ( =5%) and potential doublets expressing both female- and male-specific marker genes, initial global clustering and UMAP was performed to first separate neurons vs

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After lysis and protein quantification, equal amounts of protein were separated by SDS-PAGE, transferred to PVDF membranes and probed with specific primary and HRP-conjugated secondary antibodies

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Wein, N., Alfano, L