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After 20 h, the medium was gently removed, and the cells were examined for GFP fluorescent spots using the ImmunoSpotS6 plate reader

Everything else is protocol refinement, not risk management

Advanced Dose 4.5 mg The highest dose tested gave the most weight loss (about 11%) over 26 weeks [1]

Hypotension (may cause vasodilation through nitric oxide modulation)

With some refining, computational approaches could integrate protein, excipients, and solution physics at multiple length scales from atomistic models to solution scale

Higher doses of 1000-1200mg are sometimes used for people who want faster results or have been doing treatments for a while