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Samples (30 g protein) were resolved by 420% SDS-PAGE (Biofuraw Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)
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Two types of TSs were generated: (i) TS-1, a simple insertion of an elastic linker (GPGGA) 8 at Tyr145 of EGFP, where GFP has been shown to tolerate the insertion of the full protein 1,14 , and (ii) TS-2, cpEGFP with the same elastic linker 14 (Fig
Rotate injection sites consistently, never using the same spot twice in a row
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Human copper peptide pharmacology and wound healing
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