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241 DPP-4 cleaves GLP-1(7-36) at the 8th position, generating GLP-1(936)

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3.2 Quality assessment Risk of bias was prepared for 19 included studies

An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)