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(The supernatant can be stored at -80 for 10 days.) Cell sample Harvesting cell should not less than 106,then wash with PBS for twice (mix cell with PBS centrifuge at 600g for 10 minutes), mix precipitated cell with the volume of PBS for 3 times
doi: 10.1002/mco2.171 113 MillsELPierceKAJedrychowskiMPGarrityRWintherSVidoniSet al
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