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After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)
Sivalingam S, Larsen EL, van Raalte DH, Muskiet MHA, Smits MM, Tonneijck L, et al
Carnosine is an endogenous dipeptide that functions as a buffer in muscle cells, thereby attenuating the accumulation of acidic byproducts of muscular exertion (e.g lactate and hydrogen ions) [7]
Eur J Pharmacol (2021) 912:174604
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This makes NAC a popular supplement among athletes and fitness enthusiasts looking to gain a competitive edge
