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2.5 Sample preparation After evaluation of seizure behavior, the blood samples were collected from the hearts of all rats under deep anesthesia using 80 mg/kg ketamine with 10 mg/kg xylazine (Kitazawa et al., 2005

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T-HL: Conceptualization, Data curation, Formal Analysis, Investigation, Methodology, Software, Validation, Visualization, Writing original draft

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Fats and oils are mixed with a lye solution (sodium or potassium hydroxide) under controlled temperatures