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The supernatant was transferred to a 96-well plate and supplemented with Daily Assay Reagent (DNTB (5,5-dithios-(2-nitrobenzoic acid)) and NADPH (nicotinamide adenine dinucleotide phosphate) in GSH stock buffer)

Always follow the solvent named on the certificate of analysis

Furthermore, oncogenes are originally believed to elevate the ROS levels in cells, supported by the facts that ectopic expression of different oncogenes (e.g., Myc and KRAS) increase ROS levels in cells [6, 7]

While these methods provide excellent sensitivity and selectivity, they require costly instrumentation, trained personnel, and complex sample preparation steps 42

Montero, A

doi: 10.3389/fnut.2022.1018336 59 Capetillo-ZarateE.GraciaL.TampelliniD.GourasG