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Interestingly, the incubation with the antioxidant N-acetylcysteine (NAC) upon treatment with NP4 resulted in reduced levels of apoptosis, suggestive that the cytotoxicity of NP4 is related to intracellular redox homeostasis (Supplementary Fig
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Using stereoscopy, we obtained mutation rates of at least 90.6% at exon 2 and 93.8% at exon 3 based on a subset of 32 targeted larvae and 8 sibling controls collected from the same injection round
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