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DCFH-DA staining to detect intracellular ROS Cells were seeded into 12-well plates and DCFH-DA staining was performed when the cell density reached about 80%

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It is a synthetic modification of human GLP-1, though its development was inspired by the discovery of exendin-4, a long-acting peptide found in Gila monster saliva that led to earlier GLP-1 medications like exenatide