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In the next sections, we'll walk through how to mix your medication, discuss typical dosing ranges, and explain why you should never adjust your dose on your own

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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

doi:10.1038/nature08980

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Second, while leptin deficiency caused a reduction of expression of exons I, III, and VI in female ob/ob mice, female db/db mice exhibited decreased expression levels of exons I, III, IV, and VI