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Researchers commonly investigate DSIP in studies involving sleep regulation pathways, recovery mechanisms, stress-response signaling, neurological activity, and circadian rhythm function
The hippocampus was dissected out on ice and immediately homogenized in the lysis buffer with 1% phenylmethylsulfonyl fluoride and 1 PhosSTOP phosphatase inhibitor cocktail (Roche Applied Science, Penzberg, Germany)
Reconstitute under sterile conditions
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Interestingly, the tesofensine-treated rats also had lower levels of dopamine receptors in certain brain regions compared to both the placebo-treated rats and the rats on a restricted diet
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