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The methylcobalamin cofactor plays a role in the two- step synthesis of methionine by transferring the methyl radical from 5- methyltetrahydrofolate to the two- electron reduced cobalamin and then from the one- electron reduced methylcobalamin to homocysteine
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The next experimental step included extracting total RNA from the cells through the use of the acid guanidinium thiocyanate-phenol-chloroform extraction method
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