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One are those which are dark due to under eye bags and loss of volume and others are dark without loss of volume

also stated that MTX-induced oxidative stress may cause glomerular and tubular damage by causing MTX accumulation in the kidney (Reference Li, Abe and Yamakawa42)

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Association of Cord Plasma Biomarkers of In Utero Acetaminophen Exposure With Risk of Attention-Deficit/Hyperactivity Disorder and Autism Spectrum Disorder in Childhood