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Notes: Monitor subjects glucose levels to prevent hypoglycemia

Key Takeaways IGF-1 LR3 must be reconstituted with bacteriostatic water (0.9% benzyl alcohol) using slow-wall injection technique at 1mL per 2030 seconds to prevent protein denaturation from mechanical shear stress

a , SDS-PAGE immunoblot of Ndufs2 in isolated mitochondria from Control ( Ndufs2 fx/fx ) and Ndufs2 -KO ( Adiponectin-Cre

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