we did not collect data on hormonal status, including menstrual cycle phase or oral contraceptive use, which may influence GSH levels given the known interactions between sex hormones and oxidative stress pathways
Total protein was extracted from tissues and cells using RIPA lysis buffer containing proteinase and phosphatase inhibitors
Parkinsons Disease Program In Parkinsons Disease (PD), a very specific set of dopaminergic neurons in a region of the brain called the Substantia Nigra die, causing patients to have difficulty walking and they eventually can become immobile
Acknowledgements This project was supported by Fundao de Amparo Pesquisa e Inovao do Estado de Santa Catarina (FAPESC), Universidade do Extremo Sul Catarinense (UNESC), Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico of Brazil (CNPQ), and Fundao Coordenao de Aperfeioamento de Pessoal de Nvel Superior of Brazil (CAPES)
Because a perfectly measured dose of degraded peptide delivers nothing
Avoid freezing the solution, as freeze-thaw cycles can degrade peptide integrity in liquid formulations more than steady refrigeration