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Tryptic peptides were separated by reversed-phase chromatography with a nanoflow high-performance liquid chromatography (HPLC) (Easy nLC II, Thermo Fisher Scientific) system on an EASY-column (10 cm, 75 m, C18) using gradient elution [developed from 5% (v/v) acetonitrile, 0.1% (v/v) formic acid to 90% (v/v) acetonitrile, 0.1% (v/v) formic acid in water over 40 min at a flow rate of 300 nl/min]

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C.Bonner-WeirS

Rigorous Quality Control & Assurance GMP Standards Compliance: Consistent & reliable production

10 Adverse events were predominantly mild to moderate gastrointestinal symptoms, including nausea, vomiting, and decreased appetite, with no significant increases in hypoglycemia or cardiovascular events reported