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At the end of the study, the rats were under anesthesia with ketamine (10 %)/ xylazine (2 %) mixture (80/5 mg/kg) (Alfasan, Netherland), and 5 ml blood was collected by cardiac puncture
Three hundred patients with HFrEF hospitalized for acute heart failure within 14 days and taking diuretics equivalent to at least 40 mg furosemide were randomized to receive 1.8 mg per day of liraglutide or placebo in FIGHT [27]
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The DFb culture was maintained to the third generation/passage, and then surgical treatment was performed
Lytvyn Y, Bjornstad P, Udell JA, Lovshin JA, Cherney DZI
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