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Participants drank a sugary solution, and blood samples were collected every five minutes over a four-hour period

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The cell lysates were probed using anti-PAR 10H (Enzo Life Sciences, ALX-804-220-R100), anti-Flag M2 (Merck/Sigma, F7425), anti--tubulin (Abcam, ab179513) and anti-GFP JL-8 (Clontech/TaKaRa, 632381)