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Validation should cover sensitivity, accuracy, recovery, carryover, and precision across the expected concentration range (Thiel et al., 2023)

The Biochemical Relationship (Simplified) NAD+ metabolism produces NADPH NADPH is required to keep glutathione in its active (reduced) form Active glutathione neutralizes oxidative stress Reduced oxidative stress helps preserve NAD+ levels In other words: NAD+ helps keep glutathione working

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Regular monitoring may be appropriate for patients with chronic conditions, ensuring stability and detecting any disease progression

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