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However, for concentrations exceeding three g/dl, dilution with a 0.1 M phosphate buffer at pH 7.0 was necessary to obtain reliable results

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First, 0.4 mg/ml TGR protein was mixed with 5 mM of inhibitor 9 in buffer solution containing 0.15% DMSO and incubated 30 min at room temperature (20 C)

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