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Quantitative real-time polymerase chain reaction The total RNA was extracted from VSMCs using the TRIzol reagent (Invitrogen) and cDNA was synthesized via reversetranscription using the High-Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA, USA), according to the manufacturers instructions

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Smaller brands, like Hydrant , are promoting products that support hydration and electrolytes, which can be beneficial for individuals experiencing nausea or dehydration from GLP-1 medications