Step 6: Refrigerate immediately at 2-8C (36-46F)
To advance PRR-targeted therapies, several key priorities must be addressed: (1) Developing delivery systems that ensure accurate and efficient administration of PRR-targeted treatments is essential
Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency
Clean the injection site: Clean the skin with an alcohol swab
This is especially important for those with oily or combination skin, as harsh cleansers can trigger excess oil production as a compensatory response
Typically resolves with continued use