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Additionally, mitochondrial ROS-induced oxidation of GSDMD is a crucial mechanism facilitating GSDMD cleavage, which in turn drives NLRP3-dependent pyroptosis in macrophage [144]

ATP depletion stimulates PKC-alpha activity and leads to translocation of the enzyme to the plasma membrane, thereby enhancing serine phosphorylation of membrane proteins, such as the Ca 2+ -permeable cation channel (Fig

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in a casecontrol in vivo and in vitro study

Cells were fixed with 4% paraformaldehyde in PBS for 15 min

Nat Cell Biol 28 , 903914 (2026)