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Affinity pulldown assay The proteins and amounts indicated in the figures were incubated in a 15 l reaction buffer AP (25 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, 10% glycerol, 0.01% Igepal CA-630, 0.5 mM DTT) with 100 mM KCl, 2 mM MgCl 2 , and 8 U TurboNuclease (Accelagen, Cat#NC0298896) at room temperature for 20 min

10.1053/j.gastro.2023.12.022 65 MariaT

We have recently established Pdx1-Cre;Trp53 R172H ;Rb f/f mice, which develop endogenous well-differentiated insulinoma as well as glucagonoma with full penetrance 22